From this perch, PINK1’s kinase domain springs into action, phosphorylating ubiquitin, which recruits the ubiquitin ligase Parkin—another PD-linked protein—to tag mitochondria for degradation. While the broad strokes of this process are well-known, precisely how PINK1 remains anchored to the membranes of ailing mitochondria was a puzzle.
These parkin knockout (KO) mice were generated by creating a germline disruption in the parkin gene within exon 3 leading to a truncated transcript coding for the first 95 amino acids of mouse parkin protein fused in-frame with the coding sequence of EGFP (Goldberg et al., 2003). Loss-of-function ...
The Q311X mutation is a nonsense mutation that produces a C-terminally truncated parkin, just 155 amino acids long. Parkin-Q311X mice (line A) have two copies of the transgene (Tg) integrated in tandem in a bacterial artificial chromosome (BAC), although its integration site is unknown. Notably, as ...
Qi Y, Zhang J, Zhang Y, Zhu H, Wang J, Xu X, Jin S, Wang C, Zhang F, Zhao M, Wu Z, Zhu H, Yan P. Curcuma wenyujin extract alleviates cognitive deficits and restrains pyroptosis through PINK1/Parkin mediated autophagy in Alzheimer's disease.
This knockout (KO) rat model was created at Sage Labs (acquired first by Horizon Discovery, then by Envigo, and most recently by Inotiv) in collaboration with the Michael J. Fox Foundation. It carries a disrupted Park2 (also known as Prkn) gene, which encodes the E3 ligase parkin. Park2 mRNA levels ...
Jung YY, Son DJ, Lee HL, Kim DH, Song MJ, Ham YW, Kim Y, Han SB, Park MH, Hong JT. Loss of Parkin reduces inflammatory arthritis by inhibiting p53 degradation. Redox Biol. 2017 Aug;12:666-673. Epub 2017 Apr 5 PubMed.