TUNEL is a method for detecting apoptotic DNA fragmentation, widely used to identify and quantify apoptotic cells, or to detect excessive DNA breakage in individual cells. [3]
Explore our TUNEL assays for imaging and flow cytometry, and identify the assay designed to meet your specific experimental needs.
The TUNEL staining / TUNEL assay method relies on the enzyme terminal deoxynucleotide transferase (TdT), which attaches deoxynucleotides to the 3’-hydroxyl terminus of DNA breaks.
TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling) is a method by which a fluorophore conjugated nucleotide is enzymatically linked to the 3′ end of fragmented DNA.
The terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay detects DNA breakage by labeling the free 3ʹ-hydroxyl termini. Given that genomic DNA breaks arise during early and late stages of apoptosis, TUNEL staining ...
Do TUNEL and Other Apoptosis Assays Detect Cell Death in Preclinical ...
The classic method for TUNEL quantitation is to manually count the number of TUNEL positive (TUNEL+) nuclei in a region. This method has several disadvantages including extensive time and labor investments.
Our TUNEL assay kits have high sensitivity, quick and easy operation, and can better assist in apoptosis research related to cell function and the R&D of related drugs.
TUNEL is used to detect cells with massive amount of DNA damage, especially apoptotic cells, as DNA fragmentation is one step of the apoptotic death program. To perform the TUNEL assay, samples are first fixed, then solubilized.
Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) is an established method for detecting DNA fragments. The TACS® TdT kits contain a highly purified form of the TdT enzyme for the enzymatic incorporation of biotinylated nucleotides.