An enzyme-linked immunosorbent assay, or ELISA test, detects immune responses in the body. ELISA tests can detect hormones, bacterial antigens, and antibodies. Read on to learn how the ELISA test works and how it's used.
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Traditional ELISA typically involves chromogenic reporters and substrates that produce some observable color change to indicate the presence of antigen or analyte. Newer ELISA-like techniques use fluorogenic, electrochemiluminescent, and quantitative PCR reporters to create quantifiable signals.
ELISA is a laboratory technique that detects certain antibodies, antigens and other substances in your blood, pee or other bodily fluid. Laboratory scientists use this technique for several medical tests — from diagnosing infections to confirming pregnancy.
Learn about ELISA assays with Pierce Protein Methods. Our comprehensive guide covers principles, protocols, and applications, enabling precise and reliable results for your research.
Each ELISA measures a specific antigen, and kits for a variety of antigens are widely available. The ELISA pictured in Figure 1 is what is known as a sandwich ELISA, here two sets of antibodies are used to detect secreted products, e.g. cytokines. The method is stepwise in the order shown.