Due to the small sample amount separated in analytical HPLC, typical column dimensions are 2.1–4.6 mm diameter, and 30–250 mm length. Also HPLC columns are made with smaller adsorbent particles (1.5–50 μm in average particle size).
High-performance liquid chromatography (HPLC) is a liquid chromatography method that is used to separate and analyze components in liquid mixtures. HPLC separates the components of a mixture by passing a liquid mobile phase through a column packed with a stationary phase under high pressure.
Thomson Instruments provides HPLC instruments, columns, generators, and lab solutions for analytical and life science labs with expert support and products.
Chromatography is an analytical technique that is used to separate mixtures by distributing the components of a mixture between a stationary phase and a mobile phase. Columns are used in various types ...
HPLC is an analytical chemistry technique applied to separate, identify, and quantify semi- and non-volatile compounds in liquid samples. Industries like pharma and biopharma, environmental, and food and beverage use HPLC for the characterization, purification, and quantitation of compounds.
HPLC is an abbreviation for High Performance Liquid Chromatography. "Chromatography" is a technique for separation, "chromatogram" is the result of chromatography, and "chromatograph" is the instrument used to conduct chromatography.
Understand the HPLC and UHPLC basics, principles, and FAQs to ensure confidence in your HPLC results. Learn HPLC fundamentals now.
Theory High-Performance Liquid Chromatography (HPLC) is an invaluable analytical technique used in separating, identifying, and quantifying compounds in a mixture. HPLC is a powerful biochemical, pharmaceutical, and environmental analytical chemistry tool.