From this perch, PINK1’s kinase domain springs into action, phosphorylating ubiquitin, which recruits the ubiquitin ligase Parkin—another PD-linked protein—to tag mitochondria for degradation. While the broad strokes of this process are well-known, precisely how PINK1 remains anchored to the membranes of ailing mitochondria was a puzzle.
These parkin knockout (KO) mice were generated by creating a germline disruption in the parkin gene within exon 3 leading to a truncated transcript coding for the first 95 amino acids of mouse parkin protein fused in-frame with the coding sequence of EGFP (Goldberg et al., 2003). Loss-of-function ...
The Q311X mutation is a nonsense mutation that produces a C-terminally truncated parkin, just 155 amino acids long. Parkin-Q311X mice (line A) have two copies of the transgene (Tg) integrated in tandem in a bacterial artificial chromosome (BAC), although its integration site is unknown. Notably, as ...
Qi Y, Zhang J, Zhang Y, Zhu H, Wang J, Xu X, Jin S, Wang C, Zhang F, Zhao M, Wu Z, Zhu H, Yan P. Curcuma wenyujin extract alleviates cognitive deficits and restrains pyroptosis through PINK1/Parkin mediated autophagy in Alzheimer's disease.
Parkin Flx/Flx mice allow for conditional knockout of the Prkn gene using a Cre-loxP recombination system (Shin et al., 2011). This approach aims to overcome the developmental compensation seen in germline parkin knockouts, enabling adult-onset studies of parkin function in defined tissues (Von ...
Progress in Parkinson's disease research and therapeutic development depends on robust preclinical models, including rodent models. By organizing information related to the characterization of selected PD models, this resource conveys what is known about each one and facilitates comparison of phenotypes. This resource was created in collaboration with The Michael J. Fox Foundation (MJFF) to ...